DNA polymerase switch-mediated CRISPR/Cas system for photoelectrochemical detection of apurinic/apyrimidinic endonuclease 1

Key Points

• Photoelectrochemical detection achieved for endonuclease 1 using a novel CRISPR/Cas system, highlighting the method's sensitivity.
• The DNA polymerase switch effectively enhances detection accuracy, facilitating better identification of apurinic/apyrimidinic sites.
• Assessment using a CRISPR/Cas framework revealed promising results, particularly in detecting specific enzymatic activities.
• This work suggests that the approach may enable advancements in targeted diagnostics, but further validation in clinical settings is needed.