Unlocking the complexity of macromolecular protein heterogeneity of glycoprotein IgE via an optimized online digestion middle-down proteomics approach

Key Points

• Glycoprotein IgE heterogeneity was successfully analyzed, shedding light on its complex structure.
• In findings, 12 distinct glycoforms were identified using an optimized online digestion approach.
• Analysis involved a middle-down proteomics strategy, enhancing protein characterization and understanding.
• Highlights the potential for deeper insights into glycoproteins, paving the way for further studies on immune responses.