Successful “micro-propagation” requires avoiding microbial contamination of plant tissue cultures at every stage of growth. Bacterial contaminants (both Endophytic and epiphytic organisms) can lead to the loss of propagated plants at every stage of development. Plants infected with bacterial contamination will suffer from impaired rooting and multiplication, and may even die. Plant death results in the loss of plant tissue, which is particularly detrimental for the tissue culture of economically important plants such as potatoes and date palms. Early detection of bacterial contamination is difficult, as it is present within plant tissues, leading to the loss of plant tissue before the contamination is detected and treated. Bacterial contamination can result from various causes, including the use of improper sterilization techniques or the use of improperly sterilized equipment. To produce sterile plant cultures, various criteria must be known, such as the source of contamination and the type of pathogen. These criteria can be minimized, and sterilized plant cultures can be produced using antibiotics or other advanced techniques. It has been found that methods of preventing bacterial contamination using chemical compounds cause harm to both the transferred and growing plant tissues and to workers in plant tissue culture laboratories or fields. Therefore, nanotechnology using various types of nanoparticles has emerged as a promising new solution to this intractable and widespread problem in plant tissue cultures. Nanoparticles may serve as an alternative to antibiotics, particularly when bacteria develop resistance to existing antibiotics.
Samir et al. (Thu,) studied this question.