A codon-resolved view of subcellular translation with LOCL-TL

Key Points

• Translation patterns were analyzed in various subcellular compartments with LOCL-TL, revealing distinct codon use.
• Key evidence showed a significant variation in codon efficiency across different regions; enhanced resolution was achieved.
• Using LOCL-TL, we generated a codon-resolved view of mRNA translation at the ribosome level across cell types.
• Findings highlight the importance of subcellular localization in translation, suggesting targeted approaches for protein synthesis research.