Under new pharmaceutical science initiatives and innovation training， we address complex biological sample analysis. A comprehensive pharmaceutical experiment was designed for this purpose. It integrates pharmaceutical analysis， polymer chemistry， and analytical chemistry. Advanced molecular imprinting technology （MIT） serves as the key tool. Cholic acid， a significant endogenous metabolite， is selected as the template. Cholic acid molecularly imprinted polymers （CA-MIPs） are synthesized via precipitation polymerization. Their structure is characterized using scanning electron microscopy （SEM） and Fourier transform infrared spectroscopy （FT-IR）. The imprinting site and formation mechanism is elucidated. Adsorption performance is evaluated by high performance liquid chromatography （HPLC） and liquid chromatography-mass spectrometry （LC-MS）. Selectivity and enrichment efficiency in plasma are assessed. The experiment covers material design， synthesis， and characterization. It also includes application performance evaluation. This demonstrates high-level innovation and challenge. The practice enhances students’ innovative thinking and practical skills. It fosters social and professional responsibility. The project cultivates high-quality applied pharmaceutical talents. This represents a beneficial practice in holistic education.
JIANG et al. (Sun,) studied this question.