Engineered DNA hydrogel paper chip biosensor by cascaded hybridization chain reaction-assisted CRISPR/Cas12a system for sensitive detection of miRNA 622

Key Points

• Sensitive detection of miRNA 622 is achieved with a biosensor based on DNA hydrogel.
• The biosensor uses a CRISPR/Cas12a system enhanced by a hybridization chain reaction for improved sensitivity.
• This method presents a novel approach to studying biomolecular interactions in real-time.
• The findings support broader applications of CRISPR technology in diagnosing genetic diseases.