Geometry-dependent regulation of myogenic and osteogenic differentiation on microgeometry polystyrene substrates

Key Points

• Myogenic differentiation is significantly impacted by substrate microgeometry, influencing cellular behavior.
• Differentiation outcomes varied markedly, with specific geometries leading to increased osteogenic potential.
• Analysis across different microgeometries reveals distinct pathways for myogenic and osteogenic differentiation.
• Understanding these differences may enhance tissue engineering applications and scaffold designs.