In vivo-generated monocyte-derived dendritic cells (moDCs) play a pivotal role in inducing effective immune responses against infections and cancer due to their exceptional cross-presentation capabilities. However, the in vivo generation of moDCs during immunotherapy is constrained by the absence of safe and efficient in vivo strategies. Here, we propose a chitosan oligosaccharide-based CaCO 3 nanoparticle-loaded hydrogel (CCH) to facilitate effective in vivo monocyte-to-moDC conversion by leveraging its in-situ spatiotemporal regulation capabilities. The CCH promotes the secretion of chemokines to recruit monocytes and then targets a dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN)-pathway to facilitate the differentiation of monocytes into moDCs within a subcutaneous immune niche formed by CCH. After the removal of DC-SIGN + cells in DC-SIGN-DTR mice, the proportion of CCH-induced moDCs significantly decreased, indicating the DC-SIGN-dependent conversion of moDCs. Accordingly, the tumor cell loaded-CCH (TCH)-induced moDCs facilitate cross-presentation to prime T cells and enhance robust antitumor T cell memory responses. Thus, subcutaneous injection of TCH effectively prevent the growth of primary liver cancer and patient-derived xenograft (PDX) colorectal cancer. Moreover, TCH inhibits postoperative tumor recurrence, providing promising advancements in immunotherapy. Our CCH-based strategy provided important insights on how to in vivo induce moDCs to harness cross-presentation for anti-tumor therapy. • Chitosan-based CaCO 3 nanoparticle-loaded hydrogel (CCH) enables efficient generation of monocyte-to-moDC in vivo. • CCH recruits monocytes and promotes their differentiation via the DC-SIGN pathway. • CCH-induced moDCs exhibit cross-presentation capabilities. • Tumor cell-loaded CCH (TCH) inhibits primary liver cancer growth.
Lin et al. (Sun,) studied this question.