Going full circle: Dynamic covalent enzyme immobilisation via visually trackable boronate esters

Key Points

• This research aims to develop a dynamic covalent strategy for enzyme immobilisation that allows for recycling and waste reduction.
• Implemented reversible immobilisation using boronate esters on solid supports.
• Evaluated enzyme activity and recycling potential over multiple uses.
• Assessed waste generation compared to traditional single-use methods.
• Achieved a significant reduction in waste generated from enzyme use.
• Showed that enzymes maintained activity over multiple recycling cycles.
• Demonstrated visual tracking of immobilised enzymes through the boronate ester technique.