Purpose: This study investigated the effect of locally injected plant-derived exosomes on inflammatory cell dynamics during skin wound healing in a rat model. Materials and Methods: Twenty-four adult Wistar albino rats were randomly assigned into two groups ( n =12 each). A standardized 1-cm full-thickness incision was created on the ventral surface of the forelimbs in all animals. Group 1 served as the control with no additional treatment, while Group 2 received intradermal injections of plant-derived exosomes (Exoline Exosomes®, Abiomaterials Co., Ltd., South Korea) directly into the wound bed immediately after incision. Biopsy samples were collected under anesthesia at baseline, day 7, and day 14 post-wounding. Histological analysis using Hematoxylin and Eosin staining quantified inflammatory cell infiltration. Histological evaluation was conducted by a blinded pathologist to minimize observer bias. Results: The group treated with plant-derived exosomes exhibited a marked reduction in inflammatory cell infiltration at all observed time points compared to the control group. Histological analysis revealed that wounds in the exosome-treated animals demonstrated more organized tissue architecture and a faster resolution of the inflammatory phase. These findings suggest that exosome administration effectively modulates the local immune response, promoting accelerated wound healing. Conclusion: Intradermal injection of plant-derived exosomes significantly modulates the inflammatory phase of skin wound healing, reducing inflammatory cell infiltration and enhancing tissue regeneration. These findings support the potential of plant-based exosomes as a novel, safe, and effective therapy for promoting cutaneous wound repair.
Adel et al. (Thu,) studied this question.