The combination of freeze-fracture techniques with FE-SEM enables the visualization of CMT arrangement in plant tissues at a high resolution and across a broad area without the need for staining or extraction of cellular components. This technique is applicable to various plant tissues and allows for detailed observation of CMTs within these tissues, providing valuable insights into the role of microtubules in the division and differentiation of plant cells.
Hatano et al. (Wed,) studied this question.