Effect of italicTaohong Siwu Decoction/italic （桃红四物汤） on IL-1，IL-6，and TNF- in limb ischemia-reperfusion injury via lncRNA H19

Key Points

• The aim is to clarify how Taohong Siwu Decoction treats and improves inflammatory responses in limb ischemia-reperfusion injury through lncRNA H19.
• Conducted experiments on 78 male SD rats divided into four groups (control, model, Taohong Siwu Decoction, lncRNA H19 blocker)
• Oral administration of Taohong Siwu Decoction and intravenous injection of lncRNA H19 blocker
• Used HE staining to observe muscle morphology and pathological changes
• Quantified lncRNA H19 expression via RT-qPCR and inflammatory factors using ELISA
• The model group showed the most severe pathological damage compared to the control group
• Taohong Siwu Decoction and lncRNA H19 blocker groups exhibited reduced damage compared to the model group
• Increased expression of lncRNA H19 was observed in the model group, while Taohong Siwu Decoction and lncRNA H19 blocker groups showed decreases compared to the model group
• Inflammatory factors TNF-α, IL-1β, and IL-6 were significantly elevated in the model group, but decreased in Taohong Siwu Decoction and lncRNA H19 blocker groups compared to the model group