To comprehensively elucidate the dynamic evolution of the flavor system throughout the industrial production process of sugarcane water (SW), stage-resolved samples from activated carbon (AC), reverse osmosis (RO), and ozone (O 3 ) treatments were collected for E-Nose, E-Tongue, and HS-SPME-GC-MS analyses. A total of 46 VOCs were identified, eight marker compounds (VIP >1) were screened and six key aroma compounds were identified (OAV >1) throughout the process. Raw water (RW) exhibited a taste profile dominated by sweetness with prominent bitterness, while its aroma was characterized by nonanal (citrusy/fatty notes), methyl 2-methylbutanoate (fruity note), and acetaldehyde (pungent/grassy notes). AC treatment significantly reduced both sweetness and bitterness through its physical adsorption. RO treatment caused a “pseudo-rebound” in these taste attributes, interpreted as a matrix-associated sensor response rather than direct evidence of increased sweet/bitter compound concentrations. After AC and RO, the aroma profile was co-dominated by nonanal and methyl 2-methylbutanoate. The final O 3 treatment profoundly reshaped the aroma profile and was associated with a sweetness-dominant taste profile, accompanied by a significant increase in aldehyde compounds. The combined contribution of these volatile compounds shifted the overall aroma profile of SW toward distinct grassy, fatty, and citrusy notes. • Aldehydes were markedly enriched after O 3 treatment and dominated the final aroma-active profile. • E-Nose/E-Tongue and HS-SPME-GC-MS revealed stage-resolved flavor evolution across AC, RO and O 3 . • AC and RO mainly attenuated the original volatile fingerprint by reducing key volatiles. • E-Tongue responses showed RW sweetness with bitterness, a RO “pseudo-rebound”, and a final sweetness-dominant profile. • E-Nose fingerprints confirmed the most distinct aroma shift occurred after ozonation.
Yao et al. (Sat,) studied this question.
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