ABSTRACT Bone combines mechanical resilience with low density and the ability to repair itself when damaged. Inspired by the fascinating density‐normalized mechanical properties of bone, synthetic porous hydroxyapatite (HA)‐based materials have been introduced. However, their production typically involves sintering, which is energy‐intensive and restricts incorporation of biologically active components. Here, we introduce an enzyme‐mediated strategy to 3D print HA‐based composites that become load‐bearing within 7 days of mineralization through an energy‐efficient room‐temperature process. This is achieved by embedding alkaline phosphatase in naturally derived hydrogel microfragments that are jammed to enable direct ink writing at room temperature. To control the porosity of the mineral‐based composites, we include enzyme‐free fragments. The resulting scaffolds exhibit compressive strengths of 3.65 MPa (5.5 MPa g − 1 cm 3 specific strength) and low cytotoxicity. Through the introduction of open pores constituting up to 52 vol.% of the scaffold, we enable cells to infiltrate the scaffolds, thereby opening up new possibilities for cells to remodel them. We foresee the combination of mechanical performance, bioactivity, and energy‐efficient processing to open new avenues for bone tissue engineering and mineral repair, where broken structures have the potential to bear significant loads much faster than currently available solutions do.
Bono et al. (Fri,) studied this question.