Multi-omics analysis reveals Yishen Qutong formula promotes ferroptosis in Lewis lung cancer associated with Keap1/Nrf2/Nqo1 pathway modulation

Key Points

• The aim is to investigate how Yishen Qutong formula induces ferroptosis in Lewis lung cancer through specific molecular pathways.
• Conducted multi-omics analysis to assess biochemical interactions.
• Evaluated the role of 3-hydroxycinnamic acid as a serum component in YSQTF.
• Analyzed the expression modulation of Keap1 using various experimental techniques.
• YSQTF significantly promotes ferroptosis in Lewis lung cancer cells.
• Alterations in the Keap1/Nrf2/Nqo1 pathway were observed in response to YSQTF treatment.
• 3-hydroxycinnamic acid contributed to the regulation of Keap1 expression.