An efficient clear-native PAGE–based workflow for cryo-electron microscopy sample preparation of large protein complexes

Key Points

• The research aims to develop an efficient method for preparing large protein complexes for cryo-EM analysis from small sample volumes.
• Developed a clear-native PAGE workflow for sample preparation.
• Isolated large protein complexes from small sample volumes.
• Applied the method for cryo-electron microscopy analysis.
• Successfully isolated large protein complexes using the new method.
• Expanded the range of possible cryo-EM targets to include challenging systems.