Multiphasic droplet microfluidics platform for controlled bacteria and mammalian cell coculture

Key Points

• To address the challenges in coculturing mammalian cells and bacteria using a microfluidics approach.
• Utilized a multiphasic droplet microfluidics platform to enable controlled coculture.
• Investigated compatibility between mammalian cells and bacteria in a miniaturized environment.
• Optimized growth conditions for both cell types in microfluidic systems.
• Demonstrated enhanced spatial control in coculture conditions.
• Achieved high-throughput biological assays using the droplet platform.
• Revealed improved growth for both bacteria and mammalian cells in the designed microfluidic system.