The aim is to optimize murine models of HFpEF for better reproducibility and effectiveness in pre-clinical studies.
Induced a 2-hit model in J substrain of C57BL/6 mice.
Developed a 4-hit model to prevent aldosterone escape.
Ensured reproducibility across sexes and substrains.
Improved reproducibility of murine HFpEF models.
Optimized models effectively manage aldosterone levels across different substrains.
Abstract
These modifications ensure the 2-hit model is induced in J substrain of C57BL/6 mice. The 4-hit model prevents aldosterone escape and enhances reproducibility across sexes and substrains.