Reversed-phase high-performance liquid chromatography (RP-HPLC) is an effective method for separating complex peptide mixtures and currently the most widely used technique for peptide purification. Traditional RP-HPLC processes face challenges such as high solvent consumption and environmental issues related to waste liquid disposal. Therefore, optimizing RP-HPLC processes to improve purification efficiency and reduce environmental impact has become a focal point of current research. This article summarizes the application of RP-HPLC in peptide purification and explores several optimization strategies, including fine-tuning chromatographic conditions, the use of novel stationary phase materials, and the substitution with green solvents. Environmentally friendly alternatives such as wateralcohol systems, ionic liquids, and deep eutectic solvents are proposed as green solvent options. By integrating ultrahigh- performance liquid chromatography (UHPLC) with other technologies, such as organic solvent nanofiltration (OSN), purification efficiency can be further enhanced while reducing solvent consumption. Finally, the future development directions of RP-HPLC technology are discussed, highlighting that the combination of intelligent processes and green technologies has the potential to promote more sustainable peptide drug production.
Zhou et al. (Thu,) studied this question.
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