This study investigated the nitrogen removal characteristics and genomic features of the heterotrophic nitrification-aerobic denitrification bacterium Pseudomonas sp. JQ-H3. Genomic sequencing analysis revealed a genome size of 1. 30 Gb, comprising 22 contigs, with a Guanine and Cytosine content of 65. 4% and 4215 predicted genes. Functional annotation and metabolic pathway analysis identified key genes involved in nitrogen metabolism, including amoA, narG, napA, nirB, etc. Furthermore, the genome of JQ-H3 contains numerous gene sequences associated with lipase synthesis, such as PROKKA₀0523, PROKKA₀0655, PROKKA₀0951, PROKKA₀2885, and PROKKA₀3529. Response surface methodology (RSM) identified the optimal conditions for nitrogen removal as pH 7. 13, temperature 29. 42°C, shaking speed 140. 28 rpm, and an initial PCL concentration of 3. 12 g/L. The strain achieved removal efficiencies of 95. 3% for ammonium and 93. 7% for nitrate, with the average rates of 2. 63 and 2. 3 mg N/ (L·h), respectively, under the optimal condition designed by RSM. This work presents the first detailed genomic insight into an HN-AD bacterium capable of simultaneous nitrogen removal and PCL biodegradation.
Xiujie et al. (Sun,) studied this question.