In this study, we demonstrate an automated approach to efficiently and reproducibly manufacture perforated poly(ε-caprolactone) (PCL) solution electrospun tubular meshes designed for critically-sized bone defect repair. The workflow improves reproducibility and reduces fabrication time by 67% (8.7 vs. 2.7 h per 10 meshes). By directly electrospinning PCL onto a rotating cylindrical mandrel, seam-related discontinuities are eliminated, and subsequent use of an automated soldering iron system enables precise 1 mm perforations that promote vascular ingrowth during bone healing. Despite the decrease in mass of the new design compared with the original design (18.24 ± 1.5 mg for old vs. 11.48 ± 1.2 mg for new design), mechanical testing revealed similar resistance to lateral compression compared with semimanually assembled meshes. This is important to prevent collapse during surgical placement and injection of osteoinductive treatments. Further, eliminating surgical glue improves the manufacturing simplicity and scaffold reproducibility. Following implantation with bone morphogenic protein-2 loaded alginate, the new design performed similarly to the original: in vivo microcomputed tomography confirmed bone formation that significantly increased ( p ≤ 0.05) over 8-weeks in an established rat femoral defect model. This study provides a novel production method of tubular scaffolds with variable dimensions and flexible perforation patterns and demonstrates improvements in fabrication efficiencies and reproducibility.
O’Neill et al. (Sat,) studied this question.