Isolation and Identification of Microbes From Hospital Workers' Identification Cards and Testing Its Antimicrobial Activity Using Flower Extracts of Cassia Auriculata_ and Rosa Damascene

Hospital environments harbor diverse microbes on surfaces like ID cards, posing infection risks. This study isolated microbes from hospital workers' ID cards, identified them, and tested antimicrobial activity of Cassia auriculata and Rosa damascena flower extracts. Microbes were isolated using swab culture, identified via biochemical tests, and tested against extracts (100 mg/ml) by agar well diffusion. Staphylococcus aureus, E. coli, and Pseudomonas sp. were identified. C. auriculata_ extract showed higher inhibition zones (12-18 mm) than R. damascena_ (8-12 mm) against S. aureus and E. coli. Both extracts exhibited antimicrobial potential, suggesting their use in natural disinfectants (Kumar et al. , 2019; Sathya et al. , 2017).