Pentose phosphate pathway in sarcoidosis

Key Points

• To explore the cellular composition and metabolic characteristics in granulomas of sarcoidosis patients.
• Single-cell RNA sequencing analysis of granuloma samples from sarcoidosis patients.
• Identification of key cell types, particularly TREM2-positive macrophages.
• Examination of elevated expression of metabolic enzyme FBP1.
• Assessment of pentose phosphate pathway (PPP) activation in macrophages.
• Inhibition studies of PPP in vitro and in vivo.
• TREM2-positive macrophages play a crucial role in granuloma formation.
• FBP1 expression is significantly increased in macrophages within the granulomas.
• Activation of the pentose phosphate pathway is observed in FBP1-positive macrophages.
• Inhibition of the PPP reduces granuloma formation in both in vitro and in vivo models.