This paper introduced a sensitive determination method measurement spectrophotometric coupled with dispersive liquid-liquid micro extraction based on charge transfer complex formation between pyridoxine hydrochloride and chloranil using alkaline buffer (pH = 10.5). After that, the extraction of charge transfer complex was conducted by injection a mixture of chloroform and acetone solvents followed by spectrophotometric measurement of tiny organic droplets at λ = 535 nm. The important parameters affecting the formation of the complex and efficiency of DLLME such as concentration of chloranil, volume and pH of buffer solution, reaction time and the optimum conditions of DLLME has been studied and optimized in detail. At the optimum conditions, linearity was obtained ranging from 0.025–0.85 μg mL–1 and 0.05–0.75 μg mL–1 for dosage form and Redbull, respectively at the optimal conditions. Limit of detection of 0.011, 0.019, 0.021, 0.018 and 0.036 μg mL–1 for standard, Tablet (50 mg), Tablet (25 mg), injection and Redbull and LOQ 0.04, 0.03, 0.08, 0.06 and 0.18 were obtained, respectively. High enrichment factor of 73 was achieved, good recoveries of 96.4–102.0% were acquired. The medicinal determination of pyridoxine hydrochloride has been effectively expanded in dosage forms and Redbull.
Ahmed et al. (Fri,) studied this question.