Synthesis of a nanogap-rich three-dimensional plasmonic AuNP film for label-free detection of multiple enzymes

Key Points

• The study aims to develop a three-dimensional plasmonic AuNP film for the effective label-free detection of multiple enzymes.
• Synthesis of a three-dimensional plasmonic AuNP film.
• Construction of a Surface-Enhanced Raman Scattering (SERS) platform.
• Integration of AuNP film with ultrasmall nanogaps for enhanced detection capabilities.
• The 3D plasmonic AuNP film successfully detected multiple enzymes without labels.
• Ultrasmall nanogaps improved sensitivity and specificity in enzyme detection.