Intestinal ischemia-reperfusion injury (IIRI) is a life-threatening vascular emergency with high mortality, commonly occurring as a complication of hemorrhagic shock or acute mesenteric arterial occlusion. When revascularization is performed after this issue, injury will occur. The influence of MSC-MVs and G-CSF on IIRI was detected in this study. Fifty-six male adult albino rats were allocated into five groups: control, ischemia-reperfusion, MSC-MVs, G-CSF, and recovery. Ischemic injury was induced for one hour using micro-vascular clamp across the superior mesenteric artery at its beginning from the aorta. After that, the clamp was withdrawn to allow reperfusion for two hours. At the end of experimental period, rats were sacrificed. Jejunal tissues were processed for assessment of oxidative stress markers (MDA and SOD) as well as for histological and immune histo-chemical analysis. Ischemia-reperfusion group revealed highly statistically significant increase in tissue MDA while SOD showed the reverse, histologically by light microscopic examination; inflammatory cellular infiltration, congested blood vessels, and separated muscle fibers in muscularis mucosa were observed. Ultrastructurally, absorptive columnar cells appeared with heterochromatic nuclei, lost apical microvilli, and widen intercellular space. These findings showed more improvement in MSC-MVs group in comparison with G-CSF group while recovery group exhibited severe affection.
Abdel-Aziz et al. (Mon,) studied this question.