Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing

Key Points

• To evaluate how different nuclei isolation methods impact data quality in brain single-nucleus RNA sequencing.
• Comparison of three nuclei isolation methods for brain tissue
• Assessment of data quality metrics including nuclei yield and RNA contamination
• Utilization of a machine-assisted approach to reduce variability
• Variation in nuclei yield and RNA contamination levels based on isolation method
• Machine-assisted method provides consistent transcriptional signatures across glial and neuronal cells
• Protocol choice significantly impacts overall data quality

Abstract