ABSTRACT Obesity is a serious public health challenge, underscoring the importance of developing natural and effective interventions. This study prepared an ethanol extract from Ganoderma atrum (EEG) and evaluated its intervention effect on high‐fat diet (HFD) mice. Analysis revealed that EEG was rich in triterpenoids, fatty acids, and conjugates, as well as linoleic acids and derivatives. Administration of EEG significantly alleviated HFD‐induced weight gain and associated metabolic disturbances, such as abnormal glucose and lipid levels, along with an amelioration in liver injury. EEG intervention also modulated the gut microbiota composition, specifically raising the levels of Lachnospiraceae NK4A136group and norankf_ Lachnospiraceae, while decreasing the abundance of Limosilactobacillus and Ligilactobacillus. In addition, EEG increased fecal conjugated bile acids, particularly tauroursodeoxycholic acid (TUDCA), taurolithocholic acid (TLCA), and taurochenodeoxycholic acid (TCDCA). Further correlation analysis showed a strong negative correlation between Ligilactobacillus and conjugated bile acids. These results indicate that the anti‐obesity effects of EEG are mediated through modifications of the gut microbiota and associated bioactive metabolites.
Fang et al. (Sun,) studied this question.