Objectives Develop a high-throughput, fully automated workflow for dicentric chromosome and micronucleus assays to enable rapid, accurate radiation dose estimation in radiological or nuclear emergencies. Methods Blood from four healthy donors was irradiated (0–5.00 Gy, 137 Cs γ-rays), repaired for 2 h at 37°C, and processed via an integrated platform (Chromprep + Metafer 4 + LS-CAS). Dicentric chromosomes (per 100 cells) and micronucleus (per 1,000 cells) were automatically scored. Dose–response data fitted to a quadratic model and validated using blinded samples with 95% confidence intervals accounting for Poisson statistics and curve uncertainty. Results The automated dose-response curves for chromosome aberrations and micronuclei are y =8.4642x 2 -3.7786x+1.5439 and y =23.348x 2 +60.469x-1.6856, respectively. Calibration curves showed excellent fit ( R 2 =0.997 fordicentric chromosome; R 2 =0.996 formicronucleus). All validation doses fell within estimated confidence intervals. The system achieved end-to-end automation with minimal human intervention while maintaining high accuracy and reproducibility. Conclusion This workflow provides a standardized, scalable, and reliable solution for rapid biodosimetry in mass-casualty radiological incidents, effectively bridging laboratory capacity and real-world emergency response needs.
Wu et al. (Thu,) studied this question.