Development of herbicide-resistant peanut via prime editing and optimization of editing efficiency

Key Points

• This research aims to enhance the efficiency of prime editing to confer herbicide resistance in peanuts.
• Utilized prime editing technique to target acetolactate synthase gene.
• Introduced W574L mutation to assess its impact on herbicide resistance.
• Analyzed editing efficiency and mutation outcomes.
• Confirmed that the W574L mutation provides broad-spectrum herbicide resistance.
• Editing efficiency observed was suboptimal and highlighted for further improvement.