Protocol to design and implement scalable wireless network system for high-throughput, automated behavioral and circuit neuroscience in mice

Here, we present a protocol for implementing scalable, modular hardware and software infrastructure to remotely operate programmable miniaturized networks of wireless neural devices in mice. We describe steps for fabricating remote control module (RCM) hardware, software setup, stereotaxic surgery, and probe implantation. We then detail procedures for locomotor, food intake, and social interaction assays. These techniques help enhance automation and throughput for studies of the neurophysiological underpinnings of behavior. For complete details on the use and execution of this protocol, please refer to Qazi et al. 1 • Instructions for fabrication of wireless μ-ILED probes and remote control modules • Guidance on configuring local piconet and global internet control of the wireless system • Steps for setting up Raspberry Pi web control, scheduling commands, and logging data • Steps for implementing wireless optogenetic experiments in laboratory animals Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Here, we present a protocol for implementing scalable, modular hardware and software infrastructure to remotely operate programmable miniaturized networks of wireless neural devices in mice. We describe steps for fabricating remote control module (RCM) hardware, software setup, stereotaxic surgery, and probe implantation. We then detail procedures for locomotor, food intake, and social interaction assays. These techniques help enhance automation and throughput for studies of the neurophysiological underpinnings of behavior.