Localized catalytic hairpin assembly-based DNA tetrahedral sensor for sensitive and rapid miRNA detection in living cells

Key Points

• The aim is to develop a fast and sensitive sensor for detecting miRNA using catalytic hairpin assembly.
• Utilized catalytic hairpin assembly for signal amplification.
• Designed a DNA tetrahedral structure for enhanced detection.
• Conducted experiments in living cells to assess responsiveness.
• Achieved rapid detection of miRNA in real-time.
• Demonstrated high sensitivity to low-concentration targets.
• Showed that the sensor works effectively in living cell environments.