This work presents a computational model of excitatory neuronal networks derived from human-induced pluripotent stem cells (hiPSCs), whose activity was recorded with Micro-Electrode Arrays (MEAs). A key feature of in vitro neuronal cultures is the emergence of network bursts - population events involving most neurons, characterized by different durations, firing frequencies, and recruitment patterns. Our numerical approach investigates the mechanisms underlying these dynamics, addressing the limitations of experimental systems that make it difficult to isolate specific parameters and processes. The model aims to investigate how local neuronal dynamics and global structural connectivity interact to shape the emergence, propagation, and termination of network bursts, highlighting the interdependence between intrinsic and network-level mechanisms. We demonstrate the critical role of noise in triggering network bursts. At the same time, non-random, structured network topologies are essential for sustaining and shaping the resulting collective spatiotemporal firing patterns. In particular, we showed that the organization of incoming and outgoing degrees significantly modulates population recruitment and burst structure, with a hierarchical organization of afferent connectivity emerging as the dominant determinant of collective dynamics. By integrating in vitro observations into in silico simulations, the present study provides a solid foundation for understanding the principles governing human neuronal network function. Also, it sets the stage for investigating how alterations of network properties may contribute to pathological conditions. Significance statement We developed a computational model that replicates the spontaneous activity of neuronal networks derived from human induced pluripotent stem cells. With a build-to-understand approach, this model helps us to understand how human brain cells generate complex activity patterns and highlights the critical role of noise in triggering collective population events. Our results demonstrate how network topology influences neuronal communication and information processing. By integrating experimental observations and theoretical models, this work provides a new tool for exploring the principles of human brain function and how these processes may be disrupted in neurological conditions.
Barabino et al. (Tue,) studied this question.