Extended Characterisation of the Coding Sequence of the HLA‐DRB4*01:05 Allele by Third‐Generation Sequencing

Key Points

• The central aim is to characterize the coding sequence of the HLA-DRB4*01:05 allele.
• Utilized third-generation sequencing technology to analyze the allele's coding sequence.
• Focused on identifying nucleotide differences between related alleles.
• Identified a single nucleotide substitution in codon 81 of exon 2.
• Confirmed the genetic variation between HLA-DRB4*01:05 and HLA-DRB4*01:01:01:03.