Optimization of cryopreservation formulations for the entomopathogenic nematode Steinernema carpocapsae

Key Points

• The aim is to optimize cryopreservation formulations for the nematode Steinernema carpocapsae to ensure their viability and effectiveness.
• Developed a polyol-trehalose formulation for cryopreservation
• Assessed viability and pathogenicity of cryopreserved nematodes
• Significantly higher viability of EPNs post-cryopreservation
• Enhanced pathogenicity of cryopreserved nematodes
• Improved germplasm management for EPN-based biocontrol