Profiles of deoxynivalenol (DON), 3-acetyl-DON (3-ADON), 15-acetyl-DON (15-ADON), DON-3-glucoside (DON-3G), and nivalenol (NIV) were evaluated in wheat grown in an experimental field under different agronomic conditions regarding their roles in co-contamination with group B trichothecenes (TCTBs). For three years in southwestern Brazil, a susceptible and moderately resistant cultivar was cultivated in irrigated and rainfed systems. A randomized complete block design was carried out to evaluate the effects of the treatments: T1 (fungicide + potassium silicate (KS)), T2 (fungicide), T3 (KS), and T4 (control) on the TCTBs profile that was determined by the validated QuECHERS-HPLC-PAD method. Co-contamination occurred in 79.2% of the samples and the highest level for 15-ADON (1640 μg/kg). In 20 samples, the sum of TCTBs was above the maximum tolerable limit (MTL) for DON (1000 μg/kg). Samples from T1 and T3 had lower contamination levels. It is advisable to consider KS fertilization and include DON forms in MTL to reduce contamination risk.
Paulo et al. (Thu,) studied this question.