Liquid biopsy in oral cancer: Detection of TP53 codon 72 polymorphism in saliva

Key Points

• This research aims to evaluate the effectiveness of saliva-based liquid biopsy for genotyping TP53 codon 72 polymorphism in oral cancer.
• Collected 33 pre-surgical saliva samples and matched tissue samples.
• Extracted cell-free DNA from saliva for analysis.
• Used digital droplet PCR (ddPCR) to detect TP53 codon 72 polymorphism.
• Compared genotyping results with tissue DNA.
• Identified genotypes: C/G (45.5%), G/G (36.3%), and C/C (18.2%).
• Observed strong concordance between salivary cfDNA and tissue DNA.
• Confirmed saliva as a reliable source for TP53 genotyping.

Abstract