Isolation and characterization of blastmycetin G, a sofa-conformation fixed analog of teleocidin B’s, produced by Streptomyces blastmyceticus

Key Points

• The study aims to isolate and characterize blastmycetin G and evaluate its biological activity.
• Isolated blastmycetin G from Streptomyces blastmyceticus
• Characterized the molecular structure and conformation
• Assessed binding affinity to PKCα and PKCδ-C1 domains
• Blastmycetin G was identified as a 5-hydroxy derivative of teleocidin B
• Demonstrated weaker binding affinity (400–800 times less) compared to teleocidin B-4
• Confirmed that coma fixation in a sofa conformation results in biological inactivity.