Diagnostic accuracy of the droplet digital PCR POLE mutation test in endometrial cancer: comparison with Sanger sequencing and NGS

Key Points

• The aim is to evaluate the diagnostic accuracy of the droplet digital PCR (ddPCR) for detecting POLE mutations in endometrial cancer.
• Compared ddPCR results with Sanger sequencing and NGS for POLE mutation detection.
• Measured sensitivity and specificity of ddPCR.
• Assessed the turnaround time and cost-effectiveness of ddPCR in clinical settings.
• ddPCR showed superior sensitivity and specificity compared to Sanger sequencing.
• Excellent agreement was found between ddPCR and NGS.
• ddPCR is cost-effective and suitable for routine clinical use, potentially improving patient outcomes.