Over 20 years of very active work on DNA barcoding of various types of multicellular organisms, specific markers have been selected for many groups, primers were developed for many selected markers, DNA barcodes were obtained for more than 400 thousand species, and a special BOLD database was created. Next-generation sequencing methods allow DNA barcodes to be obtained immediately for many objects, including for various samples stored in museum collections. DNA barcode analysis reveals many previously unknown and undescribed species in various animal groups. Successes have been achieved in various areas of practical application of DNA barcodes. However, many problems and controversial issues remain, primarily in the possibility of describing new species based on DNA barcodes, as well as in the accuracy of identifying samples using reference libraries.
Shneyer et al. (Wed,) studied this question.