Intragenic and Cisgenic Enhancement of Anthocyanin Biosynthesis in Edible Crop Tissues via Tissue-Specific Expression of Endogenous R2R3-MYB Transcription Factors.

Overview This record establishes prior art for methods and compositions aimed at enhancing anthocyanin biosynthesis in the edible tissues of crop plants using intragenic or cisgenic approaches. Specifically, the disclosure details the use of genetic elements derived exclusively from the host species or its sexually compatible relatives. The primary embodiment describes a fully intragenic construct comprising a host-derived ripening promoter (e. g. , the *Solanum lycopersicum* fruit-ripening-specific E8 promoter) operably linked to a host-derived anthocyanin-activating R2R3-MYB transcription factor (e. g. , the *S. lycopersicum* ANT1 coding sequence) and a native 3' terminator. Purpose and Licensing This technical disclosure is published by the Open Source Cultivars Project (OpenCultivars. org). It is placed entirely into the public domain under the CC0 1. 0 Universal (Public Domain Dedication) license for the express purpose of establishing prior art and preventing patent encumbrance. This ensures that these intragenic and cisgenic breeding methods remain freely available to the open-source plant breeding community. Included Documents Defensive Publication (`OpenCultivarsDefensivePubAnthocyaninMYB₂0260330. pdf`): The core technical disclosure outlining the primary tomato embodiment, methods for marker-free segregation and CRISPR-mediated promoter replacement, alongside closely related cross-species variants (e. g. , pepper, eggplant, potato, and maize). Prior Art Analysis (`OpenCultivarsPriorArtAnthocyaninMYB₂0260330. pdf`): A comprehensive survey of existing patents (including US 8, 802, 925 B2) and academic publications. This document explicitly maps the prior art landscape and identifies the specific novelty gap that this fully intragenic/cisgenic strategy addresses.