A New Immunofluorescence Assay Allows the Sensitive Detection of Anti‐Cytosolic 5′‐Nucleotidase 1A Autoantibodies

Key Points

• The aim is to establish a sensitive and specific immunofluorescence assay for detecting anti-cN1A autoantibodies in inclusion body myositis.
• Developed an immunofluorescence assay utilizing GFP-cN1A for detection.
• Analyzed sera from patients diagnosed with inclusion body myositis.
• Compared sensitivity and specificity with alternative assays.
• The new assay showed higher sensitivity for detecting anti-cN1A autoantibodies than other assays.
• No loss of specificity was reported compared to alternative methods.