Advancing fish tissue reference material development for Ag nanoparticles through a perfusion approach

Key Points

• The aim is to develop reliable reference materials for measuring silver nanoparticles in biological samples.
• Utilized a perfusion approach followed by grinding and milling.
• Created a homogeneous reference material containing engineered nanomaterials (ENMs).
• Supported validation of measurements using single particle inductively coupled plasma mass spectrometry (SP-ICP-MS).
• Developed an in-house biological reference material for ENM hazard assessment.
• Achieved homogeneity in the nanoparticle-containing biological sample.