This study aimed to develop a niosome delivery system loaded with mung bean seed coat (MBSC) extract for cosmetic applications. The extract was prepared using Soxhlet extraction with 40% ethanol and demonstrated strong antioxidant activity due to its rich content of phenolic and flavonoid compounds. Twelve different formulations (F1–F12) were prepared using sonication, and their properties, such as entrapment efficiency, particle size, polydispersity index (PDI), and zeta potential, were evaluated. Formulation F12 exhibited the most favorable overall characteristics, demonstrating high entrapment efficiency for phenolics (76.12%) and flavonoids (99.64%), nanoscale particle size (286.43 ± 15.79 nm), acceptable PDI (0.38 ± 0.50), and good physical stability (zeta potential −44.10 ± 1.01 mV). FT‐IR analysis confirmed the successful encapsulation of the extract in the niosomal bilayer. Laboratory experiments demonstrated that F12 gradually released the extract and improved skin absorption over 24 h through diffusion. Additionally, HPLC testing confirmed accurate and precise measurements of vitexin and isovitexin, meeting ICH standards for sensitivity and reliability. Furthermore, in vitro studies have demonstrated that the extract effectively protects against UVB‐induced cellular damage, thereby supporting its potential application in skincare formulations. These results suggest that Formulation F12 is a safe and effective niosome system with potential for cosmeceutical applications.
Souliyanon et al. (Thu,) studied this question.