Flow-Programmable and Reversible Surface-Induced LLPS in Nanofluidic Channels

Key Points

• The aim is to explore liquid-liquid phase separation as a programmable method for concentrating biomolecules using nanofluidic channels.
• Developed flow-programmable nanofluidic channels
• Induced liquid-liquid phase separation dynamically
• Analyzed biomolecule concentration effects
• Demonstrated successful reversible liquid-liquid phase separation
• Achieved selective concentration of biomolecules in channels
• Revealed dynamic control over phase separation processes