Engineering the efficient Exo‐Cas12i2 for MITE manipulation in rice

Key Points

• The central aim is to develop Exo-Cas12i2 for efficient manipulation of MITEs in rice to improve agronomic traits.
• Developed Exo-Cas12i2 v1 by fusing T5E and PapE exonucleases.
• Applied Exo-Cas12i2 v1 to edit TA-rich genomic regions.
• Executed experiments to assess gene regulation and trait expression.
• Achieved effective deletions of large genomic fragments.
• Facilitated regulation of genes relating to cell elongation and ethylene responses.
• Demonstrated potential for generating favorable agronomic traits in rice.