Abstract Multiple retrospective studies and meta-analyses have shown that presence of lymphovascular invasion (LVI) and/or perineural invasion (PNI) is associated with significantly worse disease-free and overall survival in stage II colorectal cancer (CRC), and these features are commonly considered when deciding whether to recommend adjuvant chemotherapy for stage II disease. Current study reviewed 32 stage II CRC patient archived FFPE samples to investigate immune cell features among LVI, PNI or no-invasion groups. IHC control, multiplex immunofluorescence (mIF) panel control and the followed mIF staining were conducted using PN 7-Plex Detection Kit (PhenoVision Bio Co., Itd) targeted CD68, CD3, CD20, panCK, PD-1, PD-L1 and CD68, panCK, CD3, CD8, CD163, αSMA, respectively. The QC procedures made sure each marker from mIF results exhibited identical location and density of the same marker from IHC staining. The 64 staining slides were scanned, tumor areas were lined out by pathologist based on H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 6225.
Hu et al. (Fri,) studied this question.