Abstract 839: Single cell cloning iPSCs with the Pala single cell sorter preserves colony stemness and cell viability for high-throughput cell line engineering workflows.

Key Points

• This research aims to evaluate the effectiveness of the Pala single cell sorter in preserving the stemness and viability of iPSCs during cloning.
• Used Pala single cell sorter to dispense iPSCs and generate single cell derived clones.
• Compared results with traditional limiting dilution method for cell dispensation.
• Assessed colony health and stemness using TRA-1-60 immunofluorescence staining and clone area measurements.
• Pala sorter generated approximately 3-fold more single cells and colonies compared to limiting dilution.
• Colony health was equivalent for both methods, indicating Pala-derived clones are healthy.