Transient Receptor Potential Ankyrin 1 (TRPA1) channels are multi-modal receptors in animals for sensing noxious physical and chemical factors. They are considered promising biochemical targets for developing repellents of arthropod disease vectors and drugs to treat a variety of medical conditions including pain, inflammation, and itch. Recently, we discovered that cinnamodial (CDIAL), a natural drimane sesquiterpene produced by the medicinal plant Cinnamosma fragrans , activates mosquito TRPA1 channels and is antifeedant and repellent to Aedes aegypti . However, the selectivity of CDIAL for mosquito vs. human TRPA1 and the mechanism(s) of how CDIAL binds to TRPA1 channels are unknown. The first goal of this study was to characterize the activation of Aedes aegypti (Aa) and Homo sapiens (Hs) TRPA1 channels by CDIAL and compare the activation to that of two known TRPA1 agonists, nepetalactone and JT010. Electrophysiological measurements of AaTRPA1 and HsTRPA1 activation (when expressed heterologously in Xenopus laevis oocytes) revealed that CDIAL activated both channels in a similar concentration-dependent manner. Compared to CDIAL, nepetalactone was a less potent agonist of both AaTRPA1 and HsTRPA1, but showed stronger selectivity for AaTRPA1. JT010 did not detectably activate AaTRPA1, but potently activated HsTRPA1. The second goal of this study was to generate insights into a putative binding site of CDIAL on AaTRPA1. We mutated six Lys residues (656, 678, 681, 728, 738, 744) and/or a Cys residue (684) in the coupling domain previously hypothesized to be involved. Simultaneous mutation of the six Lys residues to Ala dampened the electrophysiological activation of AaTRPA1 by CDIAL, whereas changing Cys684 to Ser had no detectable impact. Protein modeling and molecular docking simulations predicted that CDIAL interacts with distinct binding pockets in AaTRPA1 and HsTRPA1 that nonetheless promote similar channel activation, contrasting with JT010, which engages a pocket centered on Cys621 (aligned to Cys684 in AaTRPA1) only in HsTRPA1. These findings illuminate potential species-dependent binding determinants for TRPA1 activation that may lead to the design of novel mosquito-selective repellents and/or TRPA1-targeted therapeutics. • Cinnamodial (CDIAL) similarly activated mosquito and human TRPA1 channels. • CDIAL was a more potent agonist of mosquito TRPA1 compared to nepetalactone. • CDIAL was a more potent agonist of mosquito TRPA1 compared to JT010. • Lysine residues in mosquito TRPA1 were important for its activation by CDIAL. • In docking simulations, CDIAL interacted uniquely with mosquito vs. human TRPA1.
Lee et al. (Wed,) studied this question.