What question did this study set out to answer?

The study aims to isolate and characterize neolignans from Piper crocatum and assess their antimicrobial and antibiofilm properties.

April 13, 2026

A new bicyclo3.2.1octanoids neolignan from Piper crocatum and evaluation of antimicrobial and antibiofilm activities

Key Points

The study aims to isolate and characterize neolignans from Piper crocatum and assess their antimicrobial and antibiofilm properties.
Isolation of neolignans from Piper crocatum leaves.
Structural elucidation using UV, FT-IR, NMR, ECD, and mass spectrometry.
Evaluation of antimicrobial activity against Streptococcus mutans and Candida albicans.
Assessment of biofilm reduction at varying concentrations.
Compounds 1 and 2 showed minimum inhibitory concentrations of 2.5 mg/ml against S. mutans and 1.25 mg/ml against C. albicans.
Compounds 1-4 reduced biofilm formation by over 50% at a concentration of 500 μg/ml.
The study adds to the chemical understanding of Piper crocatum and expands the variety of neolignan structures.

Abstract

Piper crocatum, traditionally used in Indonesia as a natural antiseptic, remains underexplored chemically. In this study, four neolignans were isolated from its leaves, including a new bicyclo3.2.1octanoid neolignan, 4,5-methylenedioxy-2'-acetyl-nectamazin C (2) and three known analogues: rel-(1'R,5'S,6'R,7S,8R)-Δ2',8'-6'-acetoxy-3,3',5'-trimethoxy-4,5-methylenedioxy-4'-oxo-8.1',7.5'-neolignan (1), crocatin A (3) and crocatin B (4). All compounds share the rare bicyclo3.2.1octanoid neolignane framework. Structures were elucidated using UV, FT-IR, 1D and 2D NMR, ECD, and mass spectrometry. Antimicrobial activity was evaluated against Streptococcus mutans and Candida albicans. Compounds 1 and 2 showed weak activity, with the same minimum inhibitory concentrations (MICs) of 2.5 mg/ml (5.6 µM) against S. mutans and 1.25 mg/ml (2.8 µM) against C. albicans. Meanwhile, compounds 1-4 reduced biofilm by more than 50% at a concentration of 500 μg/ml (≈1.1 mM, depending on molecular weight). These findings contribute to the chemical profile of P. crocatum and enrich the scaffold types of neolignans.

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